cd45ro apc vio770 (Miltenyi Biotec)

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Miltenyi Biotec cd45ro apc vio770
Cd45ro Apc Vio770, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cd45ro+apc/CD3-VioBlue/pmc13128693-105-12-39
Average 96 stars, based on 5 article reviews

cd45ro apc vio770 - by Bioz Stars, 2026-10

96/100 stars

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Incubation:

Article Title: HIV DNA reservoir and elevated PD-1 expression of CD4 T-cell subsets particularly persist in the terminal ileum of HIV-positive patients despite cART.
Article Snippet: PBMCs and LPMCs were then frozen in medium [90% fetal calf serum (Biochrom) and 10% dimethyl sulphoxide (Sigma-Aldrich)]. .. For analysis, thawed mononuclear cells were incubated without prior culturing with the following antibodies and appropriate isotype controls: CD3-APC-H7 (1:40, 560176; BD Biosciences, San Jose, California, USA), CD4-FITC (1:40, 555346; BD Biosciences), CD45RO APC (1:10, 130-109-430; Miltenyi Biotec, Germany), CCR7 Pe-Cy7 (1:80, 557648; BD Biosciences), CD27 PE (1:40, 560985; BD Biosciences), and PD-1 BV421 (1:20, 329920; Biolegend, San Diego, California, USA). ..

Activation Assay:

Article Title: Expansion of T memory stem cells with superior anti-tumor immunity by Urolithin A-induced mitophagy.
Article Snippet: For human samples, cells were stained as described above with Fixable Viability Dye-eF780, aCD95-BV421 (DX2; 562616), aCD62L-PE (DREG-56; 555544), aCCR7-AF700 (150503; 561143), aCD45RO-APC (UCHL; 559865), aCD45RA-FITC (HI100; 555488), aCD8-PE-CF594 (RPA-T8; 562282) and aCD4-BV786 (OKT4; 566806) from BD. .. For experiments studying activation, expansion and exhaustion of CAR T cells, cells were stained with aCD45RA-VioBlue (T6D11; 130-113-922; Miltenyi), amycCAR-FITC (SH1-26e7.1.3; 130-116-653; Miltenyi), aCD8- PerCP-Cy5.5 (RPA-T8; 560662; BD), CD62LPE-Vio770 (145/15; 130-113-621; Miltenyi), CD45RO-APC (UCHL1, 130-113-546;Miltenyi), Fixable Viability Dye-eF780; or mycCARFITC (SH1-26e7.1.3; 130-116-653; Miltenyi), aCD8- PerCP-Cy5.5 (RPA-T8; 560662; BD), aPD1- PE-Vio770 (PD1.3.1.3; 130-117- 810; Miltenyi), aTim3-APC (REA635; 130-119-781; Miltenyi) and Fixable Viability Dye-eF780. ..

Staining:

Article Title: Monocytes in leukapheresis products affect the outcome of CD19–targeted CAR T-cell therapy in patients with lymphoma
Article Snippet: .. Antibodies used for flow-cytometry For T-cell differentiation, fresh leukapheresis samples were stained with CD3 FITC (REA613), CD4 VioGreen (REA623), CD8 APC-Vio770 (REA734), CD45RO APC (REA611), CD62L PE-Vio700 (clone 145/15), CD197 VioBlue (REA546) and CD95 PE (REA738), all from Miltenyi Biotec. ..

Article Title: Selection of cross-reactive T cells by commensal and food-derived yeasts drives cytotoxic T H 1 cell responses in Crohn’s disease
Article Snippet: Up to 4 biopsies per patient were pooled and digested in 1 mL of Hanks’ balanced salt solution Ca 2+ Mg 2+ (Gibco) supplemented with 0.5% (v/v) human AB-serum, 100 IU ml −1 of penicillin, 100 μg ml −1 of streptomycin, 0.25 μg ml −1 of amphotericin B, 10 IU ml −1 of DNase I (CellSystems) and 1 mg ml −1 of collagenase type CLS IV (Biochrom) for 30 min at 37 °C while shaking. .. Cells were filtered through a 40-μm cell strainer to remove aggregates and stained with fluorochrome-conjugated antibodies to CD4-VioBlue, CD3-PE, CD8-PerCP, CD14-PerCP, CD20-PerCP, CD45RO-APC and CCR7-PE-Vio770 (all Miltenyi Biotec). ..

Article Title: Targeting TAG-72 in cutaneous T cell lymphoma.
Article Snippet: Transduction efficiency was evaluated by flow cytometry using either green fluorescent protein (GFP), Alexa Fluor® 488 or allophycocyanin (APC) AffiniPure F(ab’)2 Fragment Goat Anti-Mouse IgG, F(ab’)2 fragment specific (Jackson ImmunoResearch Laboratories, Pennsylvania, USA). .. Frequency of T cell subsets was determined by staining for CD3 fluorescein isothiocyanate (FITC, REA613), phycoerythrin (PE)-Vio®770 (REA613) or Vio®Blue (REA613), CD4 Vio®Blue (VIT4) or Vio®Green (REA623), CD7 PEVio®770 (CD7-6B7), CD8 APC (REA734), CD30 Vio®Blue (Ki-2), CC chemokine receptor (CCR)4 APC (REA279), CCR7 Vio®Blue (REA546) and CD45RO APC (REA611); all antibodies were acquired from Miltenyi Biotec (Bergisch Gladbach, Germany). .. Target antigen expression was characterized using the AlexaFluor® 488-conjugated anti-TAG-72 diabody (Avipep, Parkville, Victoria, Australia) or anti-TAG-72 (CC49) PE (Santa Cruz Biotechnology, Dallas, TX, USA).

Recombinase Polymerase Amplification:

Marker:

Article Title: An In Vitro Comparison of Costimulatory Domains in Chimeric Antigen Receptor T Cell for Breast Cancer Treatment
Article Snippet: .. For cell surface marker analysis, the following antibodies were used: CD56-PE (HCD56/Cat#130-114-551), CD4-FITC (OKT4/130-114-531), CD8-APC (SK1/130-110-679), CD45RO-APC (UCHL1/130-113-556), CD62L-FITC (DREG-56/130-112-077), CD25-APC (BC96/130-113-284) (Miltenyi Biotec, Bergisch Gladbach, Germany), PD1-FITC (EH12.2H7/329904), LAG-3-FITC (11C3C65/369308), TIM-3-APC (F38-2E2/345012), and CD3-PerCP (clone OKT3/317336) (BioLegend, San Diego, CA, USA). ..

Review